LOCUS       40924_26789        3444 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKITE301P DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Toda, H., Itoh, N.
  TITLE     Development of novel E. coli-Kocuria shuttle vector using cryptic 
            plasmid pKPAL3 from Kocuria palustris IPUFS-1 and utilization for 
            producing enantiopure (S)-styrene oxide
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Itoh N, Toda, H.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2017) Contact:Nobuya Itoh Toyama Prefectural 
            University, Biotechnology Research Center; 5180 Kurokawa, Imizu, 
            Toyama 939-0398, Japan URL :http://www.pu-toyama.ac.jp/BR/itoh/
REFERENCE   3  (bases 1 to 3444)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3444)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-AUG-2017) Contact:Nobuya Itoh Toyama Prefectural University, 
            Biotechnology Research Center"; volume: " 5180 Kurokawa, Imizu, 
            Toyama 939-0398, Japan URL :http"; pages: 
            "//www.pu-toyama.ac.jp/BR/itoh"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    339..395
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(399..415)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(637..1449)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1831..2419
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2441..3444
                     /label=pKPAL3-derived plasmid replication origin
                     /note="pKPAL3-derived plasmid replication origin"
ORIGIN
        1 ccgaccgccg ggccgaggag ctggggatcg ccaccaccac cgagttcgcg gcccaggccg
       61 gaggggcgca ggcgcgggcc cactagcacg ggccggtgaa ccccgcatga atgttggttc
      121 atgtggggtt tgcgccagct gtgtcgcagt tgccccccgc cactgttccc ggggcatgaa
      181 tcccgctaga atgaacacgg aagagcaggt ggaccccggg tcgtcaccgc acttcgggtg
      241 tgggatttat gccgattggt tttgatccca catccggacg ggccggagtg caccgcacac
      301 ccactcgcag aggagattca tcatatgacc atgattacga attcgagctc ggtacccggg
      361 gatcctctag agtcgacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt
      421 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      481 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      541 ctgaatggcg aatgcgattt attcaacaaa gccgccgtcc cgtcaagtca gcgtaatgct
      601 ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg
      661 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
      721 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
      781 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
      841 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt
      901 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
      961 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc
     1021 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
     1081 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
     1141 cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
     1201 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
     1261 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
     1321 caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
     1381 taaatcagca tccatgttgg aatttaatcg cggcttcgag caagacgttt cccgttgaat
     1441 atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga
     1501 tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttg
     1561 ttgaataaat cgaacttttg ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc
     1621 agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac tggtccacct acaacaaagc
     1681 tctcatcaac cgtggctccc tcactttctg gctggatgat ggggcgattc aggcctggta
     1741 tgagtcagca acaccttctt cacgaggcag acctctcgac ggatcgttcc actgagcgtc
     1801 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1861 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1921 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     1981 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2041 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2101 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2161 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2221 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2281 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2341 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2401 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt agatctttcg ctcgtgctgt
     2461 tctttgaggc cctgccctcc gccttgctgc cttcgtgctg tgctcgggat ctcgagcgca
     2521 acctccgacc agcctgggag ggaaggaggg agcggagggc agggcgatgc cttggacgcc
     2581 ccgaggacac ccgggcgggg tgtggtgggg acgttcaagg catcgtcaat cggtggtgcg
     2641 ggaccagccc ggtcggtcgg tcggtcgcat cggttggcca ccgttggtca cgcaccgtga
     2701 ggggtgcgtg gggtggctga caacggctca gggcgttact cctgtgcagt ggcggcatgt
     2761 gcgtgcagct ctggtcacac gctcggggca gggctgctgt ctcccgaggg cggctgtagg
     2821 gcttggcact ccttcgctgt ccggggattg ctcgacccgg tgacggcccc tggctgcacg
     2881 ctcggcaaaa gaaccggctc tgacccggga ggggccgcga aggcagtgcc tcacaagcgg
     2941 ctggcgatgt tggttcacgg ggcttcgcat cccgacgcca gggtgttccg atcgctggcg
     3001 ggtccagttg caggccgttg gcgcggcggt agactggtct cggtatttac tacagacctc
     3061 atggtaggcc cccgttggcg cgggggccta ccgcatacct aggggggcgt gtcgtcagac
     3121 acgcccccta ggtgttgtgg ggcatgaggt tcttgtcacc agcggcgcgc tgagatgagg
     3181 cgagcgggct cctcgtcaca ggatggaagt tcctcaacaa gccatccacg gaagacagag
     3241 cccgctcatg ccccacgcta agcgaagttt tctacgatgc agtagatccc gcagggtacg
     3301 taagtagaac aattcagcac atcgccagtc atgccttcac ccctagtgag gtgcgtcagc
     3361 ttttagaaac ctccggcttc aagaatattg caatgtatgg gggtttttct ttcggccccc
     3421 taactgattc aagtttcgcc atgg
//
